Paniagia, NancyGirón, RocíoGoicoechea, CarlosMartín-Fontelles, Mª IsabelBagues, Ana2024-07-262024-07-262020Paniagua N, Girón R, Goicoechea C, Martín-Fontelles MI, Bagues A. Maternal separation affects the electrophysiological properties of Aδ-fibres and nociceptive behaviours in male and female mice. Int J Dev Neurosci. 2020 Oct;80(6):538-546https://hdl.handle.net/10115/38881Aim: Early life adverse effects have been associated with an increased risk of suffering pain syndromes in adulthood. Although animal models are of great importance to study modifications of pain sensitivity, up to date the results obtained are contradicting due to the varied methodologies used. Therefore, the aim of the present study was to characterise, as a whole, possible modifications in visceral and somatic nociceptive responses in male and female ICR mice, submitted to two different protocols of maternal separation (MS), and possible modifications in the electrophysiological properties of peripheral nociceptive Aδ-primary afferents. Main methods: Male and female mice were submitted to 3 or 4-8 hr of daily MS from postnatal day (PND) 2-17 and early weaned. On PND 67 von Frey, hot plate and writhing tests were performed. Afterwards electrophysiological recordings were carried out, using the in vitro skin-saphenous nerve preparation in males. Key findings: The short separation protocol of MS did not modify nociceptive sensitivity; but when mice were separated from their dams for the long separation, mechanical pain thresholds were modified in male and female mice and visceral nociception was increased in female mice. Electrophysiological recordings showed that cutaneous Aδ-fibres were sensitised and their mechanotransduction properties were altered in both MS protocols. Significance: Although MS increases the activity and the mechanosensitivity of cutaneous Aδ-afferent fibres at both short and long periods of separation, only the longer interval of time induces nociceptive sensitivity alterations during adulthood. These results highlight the possible influence of a stress free environment during childhood to reduce nociceptive alterations in adulthood.maternal separation; mice; nociception; single-fibre recording.info:eu-repo/semantics/article10.1002/jdn.10049info:eu-repo/semantics/closedAccess