Casado, NataliaMorante-Zarcero, SoniaSierra, Isabel2024-01-192024-01-192023-12-18Casado, N., Morante-Zarcero, S., & Sierra, I. (2024). Miniaturized Analytical Strategy Based on μ-SPEed for Monitoring the Occurrence of Pyrrolizidine and Tropane Alkaloids in Honey. Journal of Agricultural and Food Chemistry, 72(1), 819-832. 10.1021/acs.jafc.3c048050021-8561https://hdl.handle.net/10115/28600This research was funded by MCIU/AEI/FEDER, UE, project number RTI2018-094558-B-I00and by the call Proyectos de Impulso a la Investigación para Jóvenes Doctores de la Universidad Rey Juan Carlos, project PROCESALK ref. M2984.Currently, the analysis of trace-level contaminants in food must be addressed following the Green Analytical Chemistry principles and with the commitment to the Sustainable Development Goals. Accordingly, a sustainable and ecofriendly microextraction procedure based on µ-SPEed® followed by ultra-high liquid chromatography coupled to ion-trap tandem mass spectrometry analysis was developed to determine the occurrence of pyrrolizidine and tropane alkaloids in honey samples. The µ-SPEed® procedure took approximately 3 min per sample, using only 100 μL of organic solvent and 300 μL of diluted sample. The method was properly validated (overall recoveries 72-100% and precision RSD values ≤15%) and its greenness was scored at 0.61 out of 1. The method was applied to different honey samples, showing overall contamination levels from 32 to 177 µg/kg of these alkaloids. Atropine was found in all the samples, whereas retrorsine N-oxide, lasiocarpine, echimidine and echimidine N-oxide were the main pyrrolizidine alkaloids in the samples analyzed.engAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Natural toxinsalkaloidsGreen analytical chemistryMicroextractionUHPLC-IT-MS/MSFood safetyinfo:eu-repo/semantics/article10.1021/acs.jafc.3c04805info:eu-repo/semantics/openAccess