Cussó, LorenaMirones, IsabelPeña-Zalbidea, SantiagoGarcía-Vázquez, VerónicaGarcía-Castro, JavierDesco, Manuel2024-01-042024-01-042014-12-01Cussó L, Mirones I, Peña-Zalbidea S, García-Vázquez V, García-Castro J, Desco M. Combination of Single-Photon Emission Computed Tomography and Magnetic Resonance Imaging to Track 111In-Oxine–Labeled Human Mesenchymal Stem Cells in Neuroblastoma-Bearing Mice. Molecular Imaging. 2014;13(10). doi:10.2310/7290.2014.000331535-3508https://hdl.handle.net/10115/28175Acknowledgments: We thank Alexandra de Francisco and Yolanda Sierra for their excellent work with the animal preparation and imaging protocols and Dr. A. Pérez-Martínez (Hospital Universitario Niño Jesús, Madrid, Spain) for providing the neuroblastoma cell line. Financial disclosure of authors: This work was funded in part by grants from Ministerio de Economía y Competitividad (PLE2009-0115), Red Tematica de Investigacion Cooperativa en Cancer (RTICC/ISCIII; RD12/0036/0027), the Madrid Regional Government (S-BIO-0204-2006–MesenCAM and P2010/BMD-2420-CellCAM), and the Ministerio de Ciencia e Innovación (CEN-20101014 and TEC-2010-21619-C04-01). Financial disclosure of reviewers: None reported.Homing is an inherent, complex, multistep process performed by cells such as human bone marrow mesenchymal stem cells (hMSCs) to travel from a distant location to inflamed or damaged tissue and tumors. This ability of hMSCs has been exploited as a tumortargeting strategy in cell-based cancer therapy. The purpose of this study was to investigate the applicability of 111In-oxine for tracking hMSCs in vivo by combining single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI). 111In-labeled hMSCs (10^6 cells) were infused intraperitoneally in neuroblastoma-bearing mice, whereas a control group received a dose of free 111In-oxine. SPECT and MRI studies were performed 24 and 48 hours afterwards. Initially, the images showed similar activity in the abdomen in both controls and hMSC-injected animals. In general, abdominal activity decreases at 48 hours. hMSC-injected animals showed increased uptake in the tumor area at 48 hours, whereas the control group showed a low level of activity at 24 hours, which decreased at 48 hours. In conclusion, tracking 111In-labeled hMSCs combining SPECT and MRI is feasible and may be transferable to clinical research. The multimodal combination is essential to ensure appropriate interpretation of the images.engAttribution-NonCommercial 4.0 Internationalhttps://creativecommons.org/licenses/by-nc/4.0/Single-Photon Emission Computed TomographyMagnetic Resonance ImagingMesenchymal Stem CellsTrackinginfo:eu-repo/semantics/article10.2310/7290.2014.00033info:eu-repo/semantics/openAccess