Mota-Martorell, NataliaJove, MarionaPradas, IreneSanchez, IsabelGómez, JoséNaudi, AlbaBarja, Gustavo2024-02-212024-02-212020-04-20Natalia Mota-Martorell, Mariona Jove, Irene Pradas, Isabel Sanchez, José Gómez, Alba Naudi, Gustavo Barja, Reinald Pamplona, Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity, Redox Biology, Volume 34, 2020, 101539, ISSN 2213-2317, https://doi.org/10.1016/j.redox.2020.101539.2213-2317https://hdl.handle.net/10115/30584Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Complex I; Droplet digital PCR; Longevity; Mammals; Mitochondria; NDUFV2 subunit; NDUFS4 subunit; VDAC; Western blotinfo:eu-repo/semantics/article10.1016/j.redox.2020.101539info:eu-repo/semantics/openAccess