Herranz- Jusdado, JGMorel, EOrdás, MCMartín, DDocando, FGonzález, LSanjuán, EDíaz- Rosales, PSaura, MFouz, BTafalla, C2024-05-212024-05-212023-08-06J.G. Herranz-Jusdado, E. Morel, M.C. Ordás, D. Martín, F. Docando, L. González, E. Sanjuán, P. Díaz-Rosales, M. Saura, B. Fouz, C. Tafalla, Yersinia ruckeri infection activates local skin and gill B cell responses in rainbow trout, Fish & Shellfish Immunology, Volume 140, 2023, 108989, ISSN 1050-4648, https://doi.org/10.1016/j.fsi.2023.108989.1050-4648 (online)https://hdl.handle.net/10115/33013Teleost fish lack organized structures in mucosal tissues such as those of mammals, but instead contain dispersed B and T cells with the capacity to respond to external stimuli. Nonetheless, there is still a great lack of knowledge regarding how B cells differentiate to plasmablasts/plasma cells in these mucosal surfaces. To contribute to a further understanding of the mechanisms through which fish mucosal B cells are activated, in the current study, we have studied the B cell responses in the skin and gills of rainbow trout (Oncorhynchus mykiss) exposed to Yersinia ruckeri. We have first analyzed the transcription levels of genes related to B cell function in both mucosal surfaces, and in spleen and kidney for comparative purposes. In a second experiment, we have evaluated how the infection affects the presence and size of B cells in both skin and gills, as well as the presence of plasmablasts secreting total or specific IgMs. The results obtained in both experiments support the local differentiation of B cells to plasmablasts/plasma cells in the skin and gills of rainbow trout in response to Y. ruckeri. Interestingly, these plasmablasts/plasma cells were shown to secrete specific IgMs as soon as 5 days after the exposure. These findings contribute to a further understanding of how B cells in the periphery respond to immune stimulation in teleost fish.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/article10.1016/j.fsi.2023.108989info:eu-repo/semantics/openAccess