Baena, EugeniaCunha, Sandra RMaravic, TatjanaComba, AllegraPaganelli, FedericaAlessandri-Bonetti, GiulioCeballos, LauraTay, Franklin RBreschi, LorenzoMazzoni, Annalisa2024-01-172024-01-172020Baena E, Cunha SR, Maravić T, Comba A, Paganelli F, Alessandri-Bonetti G, Ceballos L, Tay FR, Breschi L, Mazzoni A. Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems. Mar Drugs. 2020 May 18;18(5):263. doi: 10.3390/md18050263. PMID: 32443628; PMCID: PMC7280998.1600-3397https://hdl.handle.net/10115/28526The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 seconds) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin-dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.engAttribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/Odontologíachitosanmatrix metalloproteinasesbond strengthcross-linkerszymographyinfo:eu-repo/semantics/article10.3390/md18050263info:eu-repo/semantics/openAccess