Piera, AlejandroEspada, Juan J.Morales, VictoriaRodríguez, RosalíaVicente, GemmaBautista, Luis Fernando2024-09-232024-09-232024-07-30Alejandro Piera, Juan J. Espada, Victoria Morales, Rosalía Rodríguez, Gemma Vicente, Luis Fernando Bautista, Optimised phycoerythrin extraction method from Porphyridium sp. combining imidazolium-based ionic liquids, Heliyon, Volume 10, Issue 14, 2024, e34957, ISSN 2405-8440, https://doi.org/10.1016/j.heliyon.2024.e349572405-8440 (online)https://hdl.handle.net/10115/39731Phycoerythrin (PE) extraction from Porphyridium sp. was studied employing ultrasound-assisted extraction combined with aqueous mixtures of two imidazolium-based ionic liquids (ILs) simultaneously, marking a significant novelty. A face-centred central composite design and response surface optimised PE yield (EPE), considering the effects of ionic liquid concentration (IL), [Emim][EtSO4]/[Bmim][EtSO4] mass ratio (E/B), biomass concentration (BM), and time (t). Improvements in EPE by 300 % and 115 % were achieved compared to a phosphate buffer solution and the freeze-thaw method, respectively. Temperature and pH effects were examined independently, leading to the determination of optimal operating conditions: BM = 10 mg mL−1, IL = 18.6 wt%, E/B = 0.78/0.22, t = 10 min, T = 35 °C, and pH = 7.5. Results indicated the potential for reusing the ILs for at least five consecutive extraction cycles, maintaining an EPE of 94.2 % compared to fresh ones. This underscores the success and innovation of the developed technology in enhancing PE extraction from Porphyridium spengAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/PhycoerythrinIonic liquidsPorphyridium sp.Ultrasound-assisted extractionResponse surface methodologyMicroalgaeinfo:eu-repo/semantics/article10.1016/j.heliyon.2024.e34957info:eu-repo/semantics/openAccess