Seoane, PedroEspigares, MarinaCarmona, RosarioPolonio, ÁlvaroQuintana, JuliaCretazzo, EnricoBota, JosefinaPérez-García, AlejandroAlché, Juan de DiosGómez, LuisClaros, M Gonzalo2023-12-122023-12-122018-11-20Seoane P, Espigares M, Carmona R, Polonio Á, Quintana J, Cretazzo E, et al. TransFlow: a modular framework for assembling and assessing accurate de novo transcriptomes in non-model organisms. BMC bioinformatics. 2018;19 Suppl 14:416.https://hdl.handle.net/10115/27136This work was supported by co-funding by the European Union through the European Regional Development Fund (ERDF) 2014-2020 “Programa Operativo de Crecimiento Inteligente” together with Spanish AEI “Agencia Estatal de Investigación” (BFU2016-77243-P, RTC-2015-4181-2, RTC-2016-4824-2, AGL2013-41939-R and AGL2016-76216-C2-1-R), AEI-INIA (RTA2013-00023-C02 and RTA2013-00068-C03), and Junta de Andalucía (P2011-CVI-7487), as well as CSIC grant 201540E065.The advances in high-throughput sequencing technologies are allowing more and more de novo assembling of transcriptomes from many new organisms. Some degree of automation and evaluation is required to warrant reproducibility, repetitivity and the selection of the best possible transcriptome. Workflows and pipelines are becoming an absolute requirement for such a purpose, but the issue of assembling evaluation for de novo transcriptomes in organisms lacking a sequenced genome remains unsolved. An automated, reproducible and flexible framework called TransFlow to accomplish this task is described.engAttribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/article10.1186/s12859-018-2384-yinfo:eu-repo/semantics/openAccess