Casado, NataliaPerestrelo, RosaL. Silva, CatarinaSierra, IsabelS. Câmara, José2024-01-302024-01-302019-04-10Food Chemistry 292 (2019) 14–20308-81461873-7072https://hdl.handle.net/10115/29218The authors acknowledge FCT-Fundação para a Ciência e a Tecnologia (projects PEst-OE/QUI/UI0674/2019, CQM, Portuguese Government funds), and Madeira 14-20 Program, project PROEQUIPRAM – Reforço do Investimento em Equipamentos e Infraestruturas Científicas na RAM (M1420-01-0145-FEDER-000008) and ARDITI-Agência Regional para o Desenvolvimento da Investigação Tecnologia e Inovação, through the project M1420-01-0145-FEDER000005 – Centro de Química da Madeira – CQM+ (Madeira 14-20). Natalia Casado would like to thank the Rey Juan Carlos University for providing her a mobility grant to perform a pre-doctoral stay in the CQM – Centro de Química da Madeira. Rosa Perestrelo and Catarina Silva acknowledge the FCT-Fundação para a Ciência e a Tecnologia through the pos-Doc grant (SFRH/BPD/97387/2013), PhD grant (SFRH/BD/97039/2013).In this study, two different high-throughput microextraction techniques, microextraction by packed sorbents (MEPS) and micro solid phase extraction (μ-SPEed®), were evaluated and compared, regarding the performance criteria, for the isolation of polyphenols from baby foods prior to their determination by ultrahigh pressure liquid chromatography (UHPLC). To achieve the best performance, influential parameters affecting extraction efficiency (including type of sorbent, number of extraction cycles, pH, elution solvent and elution volume) were systematically studied and optimized. To enable an effective comparison, selectivity, linear dynamic range, method detection (LODs) and quantification limits (LOQs), accuracy, precision and extraction yields, were determined and discussed for both techniques. Both methods provided the analytical selectivity required for the analysis of polyphenols in baby foods. However, μ-SPEed® sample treatment in combination with UHPLC-PDA has demonstrated to be more sensitive, selective and efficient than MEPS. Appropriate linearity in solvent and matrix-based calibrations, very low LODs and LOQs, ranging between 1.37 and 13.57 μg kg−1 and 4.57 – 45.23 μg kg−1, respectively, suitable recoveries (from 67 to 97%) and precision (RSD values < 5%) were achieved for the selected analytes by μ-SPEed®/UHPLC-PDA. Finally, the validated methodologies were applied to different commercial baby foods. Gallic acid, chlorogenic acid, epicatechin, ferulic acid, rutin, naringenin and myricetin are the most dominant polyphenols present in the studied baby food samples. The proposed methodology revealed a promising approach to evaluate the nutritional quality of this kind of products.engAttribution-NonCommercial-NoDerivs 4.0 Internationalhttps://creativecommons.org/licenses/by-nc-nd/4.0/ComparisonMEPSμ-SPEedPolyphenolsBaby foodsUHPLC-PDAinfo:eu-repo/semantics/article10.1016/j.foodchem.2019.04.038info:eu-repo/semantics/openAccess