Santamaría‐Díaz, NoeliaMendez‐Arriaga, Jose ManuelSalas, Juan ManuelGalindo, Miguel A2024-02-072024-02-072016-05-17Angew. Chem. Int. Ed. 2016, 55, 6170 –61741433-78511521-3773https://hdl.handle.net/10115/29872The oligonucleotide d(TX)9, which consists of an octadecamer sequence with alternating non-canonical 7-deazaadenine (X) and canonical thymine (T) as the nucleobases, was synthesized and shown to hybridize into double-stranded DNA through the formation of hydrogen-bonded Watson–Crick base pairs. dsDNA with metal-mediated base pairs was then obtained by selectively replacing W-C hydrogen bonds by coordination bonds to central silver(I) ions. The oligonucleotide I adopts a duplex structure in the absence of Ag+ ions, and its stability is significantly enhanced in the presence of Ag+ ions while its double-helix structure is retained. Temperature-dependent UV spectroscopy, circular dichroism spectroscopy, and ESI mass spectrometry were used to confirm the selective formation of the silver(I)-mediated base pairs. This strategy could become useful for preparing stable metallo-DNA-based nanostructures.enginfo:eu-repo/semantics/article10.1002/anie.201600924info:eu-repo/semantics/restrictedAccess