Barón , MartaMorales, VictoriaFuentes, VictoriaLinares, MaríaEscribano, NuriaCeballos, Laura2025-01-142025-01-142020-01-26Barón, M., Morales, V., Fuentes, M. V., Linares, M., Escribano, N., & Ceballos, L. (2020). The influence of irrigation solutions in the inorganic and organic radicular dentine composition. Australian Endodontic Journal, 46(2), 217-225. https://doi.org/10.1111/aej.123951329-1947https://hdl.handle.net/10115/55797The aim of this study was to analyse changes in radicular dentine composition after different irrigation regimes using attenuated total reflectance Fouriertransform infrared spectroscopy (ATR-FTIR). Dentine slices from human single-root teeth were immersed in: (i) 5.25% sodium hypochlorite (NaOCl) for 1, 5 and 20 min; (ii) 17% EDTA for 1 min; and (iii) 5.25% NaOCl for 20 min, and 17% EDTA and 5.25% NaOCl, both for 1 min. Carbonate/mineral, Amide I/mineral and Amide III/CH2 ratios were determined using ATR-FTIR before and after immersion in the irrigation solutions tested. Results showed that 5.25% NaOCl significantly decreased carbonate/mineral, Amide I/mineral and Amide III/CH2 ratios (P < 0.05). Application of 17% EDTA produced no change in carbonate/mineral ratio (P = 0.120), while an increase in Amide I/mineral in apical third (P = 0.002) and Amide III/CH2 (P < 0.001) was observed. The combination of NaOCl, EDTA and NaOCl increased carbonate/mineral ratio in coronal third (P = 0.037), and Amide I/mineral (P = 0.003) and Amide III/CH2 (P = 0.001) ratios. In conclusion, irrigation solutions tested significantly affected radicular dentine composition.endentineethylenediaminetetraacetic acidFourier-transform infraredsodium hypochloritespectroscopyArticledoi: 10.1111/aej.12395info:eu-repo/semantics/closedAccess