Examinando por Autor "Ardiles, Perla R."

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Copper-functionalized nanostructured silica-based systems: Study of the antimicrobial applications and ROS generation against gram positive and gram negative bacteria
(Elsevier, 2019-11-19) Díaz-García, Diana; Ardiles, Perla R.; Díaz-Sánchez, Miguel; Mena-Palomo, Irene; del Hierro, Isabel; Prashar, Sanjiv; Rodríguez-Diéguez, Antonio; Paulina L., Páez; Gómez-Ruiz, Santiago
A series of copper-functionalized SBA-15 (Santa Barbara Amorphous) materials containing the ligands triethoxysilylpropylmaleamic acid (maleamic) or triethoxy-3-(2-imidazolin-1-yl)propylsilane (imidazoline) have been prepared. The nanostructured silica-based systems SBA-maleamic, SBA-imidazoline, SBA-maleamic-Cu and SBA-imidazoline-Cu were characterized by several methods observing that the functionalization took place mainly inside the pores of the mesoporous system. The antimicrobial behaviour of the synthesized materials against Staphylococcus aureus and Escherichia coli was tested observing a very potent activity of the copperfunctionalized systems (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for SBA-maleamic-Cu of ca. 31.25 μg/mL, which correspond with ca. 1.13 μg/mL of Cu). A study of the oxidative stress promoted by the synthesized materials showed that the SBA-maleamic-Cu and the SBAimidazoline- Cu were able to increase the reactive oxygen species (ROS) production in S. aureus by 427% and 373%, respectively, while this increase was slightly lower in E. coli (387 and 324%, respectively). Furthermore, an electrochemical study was carried out in order to determine if these materials interact with lysine or alanine to validate a potential antimicrobial mechanism based on the inhibition of the synthesis of the peptidoglycan of the bacterial wall. Finally, these studies were also performed to determine the potential interaction of the copper-containing materials with glutathione in order to assess if they are able to perturb the metabolism of this tripeptide.
Hybrid silica materials functionalized with chloroxine-based metal complexes: Exploring synergistic antibacterial activity
(Elsevier, 2025-05) García-Valdivia, Antonio A.; Zabala-Lekuona , Andoni; Ardiles, Perla R.; Páez, Paulina L.; Díaz-García, Diana; García-Almodóvar, Victoria; Gómez-Ruiz, Santiago
This study presents a novel strategy for developing advanced antibacterial materials by integrating coordination compounds with silica nanoparticles. Two new coordination compounds based on chloroxine, namely, {[Ni(chloroxine)2(H2O)2]·H2O} (1) and {[Zn(chloroxine)2(H2O)]·H2O} (2), were synthesized and subsequently used to functionalize mesoporous silica (SBA-15 and MSN) to create hybrid materials: SBA-(1)-Ni, SBA-(2)-Zn, MSN-(1)-Ni, and MSN-(2)-Zn. The Zn-based hybrids exhibited exceptional luminescence, while the Ni-based counterparts displayed the expected temperature-dependent magnetic susceptibility according to the loaded Ni amount. Antibacterial assessments against Escherichia coli and Staphylococcus aureus demonstrated a remarkable enhancement—up to 200% greater efficacy than free chloroxine, while MSN-(2)-Zn achieved the most potent minimum inhibitory concentration (MIC) of 3.96 μg/mL, demonstrating their multifunctional potential. These hybrid materials not only enhance antibacterial performance at lower drug concentrations but also offer a promising approach to combat bacterial resistance by enhancing the synergistic properties of silica and coordination compounds. This work encourages further investigation of the next generation of multifunctional antimicrobial materials based on nanomaterials and metallodrugs, with superior applications in biomedicine and nanotechnology.
Preparation and study of the antibacterial applications and oxidative stress induction of copper maleamate-functionalized mesoporous silica nanoparticles
(MDPI, 2019-01-14) Díaz-García, Diana; Ardiles, Perla R.; Prashar, Sanjiv; Rodríguez-Diéguez, Antonio; Páez, Paulina L.; Gómez-Ruiz, Santiago
Mesoporous silica nanoparticles (MSNs) are an interesting class of nanomaterials with potential applications in different therapeutic areas and that have been extensively used as drug carriers in different fields of medicine. The present work is focused on the synthesis of MSNs containing a maleamato ligand (MSN-maleamic) and the subsequent coordination of copper(II) ions (MSN-maleamic-Cu) for the exploration of their potential application as antibacterial agents. The Cu-containing nanomaterials have been characterized by different techniques and the preliminary antibacterial effect of the supported maleamato-copper(II) complexes has been tested against two types of bacteria (Gram positive and Gram negative) in different assays to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biological results showed a moderate antibacterial activity against Escherichia coli which motivated a more detailed study of the antibacterial mechanism of action of the synthesized maleamate-containing nanosystems and whose findings showed oxidative stress generation in bacterial cells. All the prepared nanomaterials were also tested as catalysts in the “solvent free” selective oxidation of benzyl alcohol, to observe if there is a potential correlation between the catalytic oxidation capacity of the materials and the observed oxidative stress in bacteria. This may help in the future, for a more accurate rational design of antibacterial nanosystems, based on their observed catalytic oxidation activity.