Examinando por Autor "Ceballos, Laura"

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Deep caries and pulp exposures management preferences in permanent teeth: A survey amongst Spanish dentists
(Wiley, 2024-10-30) Bailleul, Laura; Ceballos, Laura; Doméjean, Sophie; Fuentes, Victoria
Aim To assess Spanish dentists' management preferences for deep caries removal and exposed pulps in permanent teeth. Methodology A web-based open and anonymous survey was distributed by social media and a specific website for this project amongst dentists practicing in Spain. The questionnaire comprised 40 questions, divided into five sections: (1) demographic data and professional activity; (2) carious tissue removal; (3) decision-making regarding pulp exposure; (4) direct pulp capping and (5) pulpotomy procedures in permanent teeth. Results were descriptively analysed. Logistic regression (95% CI) analyses and X2 tests were carried out. Results A total of 538 responses were received. Half the respondents (53.7%) preferred to perform complete caries excavation for shallow and moderate carious dentin lesions, and selective excavation to firm dentin for deep lesions (57.8%). Selective removal to soft dentin and stepwise removal were much less indicated (15.4% and 10.9%, respectively). Exposed pulps in asymptomatic teeth were treated by direct pulp capping (over 80%), decreasing in the presence of reversible pulpitis symptoms (57.1%). If irreversible pulpitis was diagnosed, a pulpectomy would be performed by 53.5% and 89.9% of the respondents in, respectively, immature and mature teeth. Pulpotomy was performed routinely only by 26.4% of the clinicians. Patients' attitudes and priorities were the most relevant criteria when performing direct pulp capping and pulpotomy, together with the history of pain and the presence of bleeding. Regarding the clinical procedure, dry cotton was preferred to obtain haemostasis and Biodentine was the material of election. Conclusions Caries removal preferences and management of pulp exposure by dentists practicing in Spain deviated from vital pulp treatment guidelines, mainly regarding indications and case selection. Pulp exposure was managed by direct pulp capping in asymptomatic cases, whilst immature permanent molars favoured the indication of pulpotomy when pulpitis was diagnosed. Most clinicians used hydraulic calcium silicate cement, specifically Biodentine, to perform vital pulp treatments. Postgraduate formation and continuing education in caries lesions management and vital pulp treatments were consistently related to more conservative and updated decisions
Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems
(MDPI, 2020) Baena, Eugenia; Cunha, Sandra R; Maravic, Tatjana; Comba, Allegra; Paganelli, Federica; Alessandri-Bonetti, Giulio; Ceballos, Laura; Tay, Franklin R; Breschi, Lorenzo; Mazzoni, Annalisa
The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 seconds) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin-dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.
Endogenous Enzymatic Activity in Dentin Treated with a Chitosan Primer
(MDPI, 2021) Maravic, Tatjana; Baena, Eugenia; Mazzitelli, Claudia; Uros, Josic; Mancuso, Edoardo; Checchi, Vittorio; Generali, Luigi; Ceballos, Laura; Breschi, Lorenzo; Mazzoni, Annalisa
The aim of this study was to evaluate the effect of different concentrations of chitosan polymer on dentinal enzymatic activity by means of gelatin and in situ zymography. Human dentin was frozen and ground in a miller. Dentin powder aliquots were demineralized with phosphoric acid and treated with three different concentrations of lyophilized chitosan polymer (1, 0.5 and 0.1 wt%) dissolved in distilled water. Dentin proteins were extracted from each experimental group and electrophoresed under non-reducing conditions in 10% SDS-PAGE containing fluorescein-labeled gelatin. After 48 h in the incubation buffer at 37 °C, proteolytic activity was registered under long-wave UV light scanner and quantified by using Image J software. Furthermore, additional teeth (n = 4) were prepared for the in situ zymographic analysis in unrestored as well as restored dentin pretreated with the same chitosan primers. The registered enzymatic activity was directly proportional to the chitosan concentration and higher in the restored dentin groups (p < 0.05), except for the 0.1% chitosan primer. Chitosan 0.1% only showed faint expression of enzymatic activity compared to 1% and 0.5% concentrations. Chitosan 0.1% dissolved in water can produce significant reduction in MMPs activity and could possibly contribute to bond strength preservation over time.
Graphene/silk fibroin scaffolds enhance dental pulp stem cell osteo/odontogenic differentiation
(2023-12-19) López-García, Sergio; Aznar-Cervantes, Salvador D; Pagán, Ana; Llena , Carmen; Forner, Leopoldo; Sanz, José L; García-Bernal, David; Sánchez-Bautista, Sonia; Ceballos, Laura; Fuentes, Victoria; Melo, María; Rodríguez-Lozano, Francisco J; Oñate-Sánchez, Ricardo E
Objectives The current in vitro study aims to evaluate silk fibroin with and without the addition of graphene as a potential scaffold material for regenerative endodontics. Material and Methods Silk fibroin (SF), Silk fibroin/graphene oxide (SF/GO) and silk fibroin coated with reduced graphene oxide (SF/rGO) scaffolds were prepared (n = 30). The microarchitectures and mechanical properties of scaffolds were evaluated using field emission scanning electron microscopy (FESEM), pore size and water uptake, attenuated total reflectance fourier transformed infrared spectroscopy (ATR-FTIR), Raman spectroscopy and mechanical compression tests. Next, the study analyzed the influence of these scaffolds on human dental pulp stem cell (hDPSC) viability, apoptosis or necrosis, cell adhesion, odontogenic differentiation marker expression and mineralized matrix deposition. The data were analyzed with ANOVA complemented with the Tukey post-hoc test (p < 0.005). Results SEM analysis revealed abundant pores with a size greater than 50 nm on the surface of tested scaffolds, primarily between 50 nm and 600 µm. The average value of water uptake obtained in pure fibroin scaffolds was statistically higher than that of those containing GO or rGO (p < 0.05). ATR-FTIR evidenced that the secondary structures did not present differences between pure fibroin and fibroin coated with graphene oxide, with a similar infrared spectrum in all tested scaffolds. Raman spectroscopy showed a greater number of defects in the links in SF/rGO scaffolds due to the reduction of graphene. In addition, adequate mechanical properties were exhibited by the tested scaffolds. Regarding biological properties, hDPSCs attached to scaffolds were capable of proliferating at a rate similar to the control, without affecting their viability over time. A significant upregulation of ALP, ON and DSPP markers was observed with SF/rGO and SF/GO groups. Finally, SF/GO and SF/rGO promoted a significantly higher mineralization than the control at 21 days. Significance Data obtained suggested that SF/GO and SF/rGO scaffolds promote hDPSC differentiation at a genetic level, increasing the expression of key osteo/odontogenic markers, and supports the mineralization of the extracellular matrix. However, results from this study are to be interpreted with caution, requiring further in vivo studies to confirm the potential of these scaffolds.
Influence of repair procedure on composite-to-composite microtensile bond strength
(Mosher & Linder Inc, 2015) Baena, Eugenia; Vignolo, Valeria; Fuentes, María Victoria; Ceballos, Laura
Purpose: To investigate the effect of different repair procedures and storage time on microtensile bond strength (μTBS) of a resin composite to an older one from a simulated previous restoration. Methods: Composite disks were made by layering 2 mm-thick increments of a nanohybrid composite (Grandio) shade A1 in a Teflon mold (4 x 8 mm). Afterwards, they were light-cured and stored (37 degrees C/7 days) in a saline solution. Specimens were randomly divided into groups according to the surface treatment applied: (1) Composite surface was roughened with a bur (Cimara) and Solobond Plus adhesive was applied; (2) Sandblasting with 27 μm aluminum oxide particles (KaVo Rondoflex), and adhesive application; (3) Air-abrasion with 30 μm alumina particles coated with silica (CoJet Sand), silane (Monobond-S) and adhesive application; (4) Negative control group with only adhesive application. Afterwards, Grandio composite (shade A3.5) was packed incrementally on the treated surface obtaining another disk (4 x 8 mm). Repaired blocks were stored (24 hours or 6 months) and afterwards μTBS test was performed and failure mode was evaluated. Also, beams obtained from 8 mm-high composite blocks without any surface treatment were immediately submitted to μTBS test to determine Grandio composite cohesive bond strength (positive control group). Data were analyzed using ANOVA and Tukey's test (P < 0.05). Results: The repair procedure affected μTBS values (P < 0.001) while neither storage time nor interactions did (P > 0.05). All repair procedures achieved bond strength values higher than the negative control group but they did not reach the composite's cohesive bond strength. The overall conclusion was that an increased superficial roughness by means of a bur, silica coating or alumina sandblasting improved μTBS of the repaired composite and bond strength remained stable after 6 months.
L‑arginine‑containing mesoporous silica nanoparticles embedded in dental adhesive (Arg@MSN@DAdh) for targeting cariogenic bacteria
(BioMed Central, 2022-12-01) López Ruíz, Marta; Navas, Francisco; Fernández García, Paloma; Martínez Erro, Samuel; Fuentes, María Victoria; Giráldez, isabeI; Ceballos, Laura; Ferrer Luque, Carmen María; Ruíz Linares, Matilde; Morales, Victoria; Sanz, Raúl; García Muñoz, Rafael Á.
Dental caries is the major biofilm-mediated oral disease in the world. The main treatment to restore caries lesions consists of the use of adhesive resin composites due to their good properties. However, the progressive degradation of the adhesive in the medium term makes possible the proliferation of cariogenic bacteria allowing secondary caries to emerge. In this study, a dental adhesive incorporating a drug delivery system based on L-arginine-containing mesoporous silica nanoparticles (MSNs) was used to release this essential amino acid as a source of basicity to neutralize the harmful acidic conditions that mediate the development of dental secondary caries. The in vitro and bacterial culture experiments proved that L-arginine was released in a sustained way from MSNs and diffused out from the dental adhesive, effectively contributing to the reduction of the bacterial strains Streptococcus mutans and Lactobacillus casei. Furthermore, the mechanical and bonding properties of the dental adhesive did not change significantly after the incorporation of L-arginine-containing MSNs. These results are yielding glimmers of promise for the costeffective prevention of secondary caries.
Long-Term In Vitro Adhesive Properties of Two Universal Adhesives to Dentin
(MDPI, 2023-04-28) Hurtado, Ana; Fuentes, Victoria; Cura, María; Tamayo, Ana; Ceballos, Laura
The composition of universal adhesives, as well as the adhesive strategy, may influence bonding effectiveness and durability. This study aimed to evaluate the microtensile bond strength (µTBS) and nanoleakage (NL), immediately and after 6-month aging, and in situ degree of conversion (DC), of two universal adhesives (Scotchbond Universal Adhesive, SBU; Xeno Select, XEN) applied with etch-and-rinse (ER) and self-etch (SE) strategies, in comparison with a two-step SE adhesive (Clearfil SE Bond, CSE). Dentin surfaces of fifty human third molars were randomly assigned to the following adhesives: two universal adhesives, SBU and XEN, applied in ER or SE mode and CSE, used as control. Teeth were sectioned into beams to be tested under µTBS, half of them after 24 h, and the rest after 6 months of water aging. Selected beams from each tooth were used for NL evaluation and in situ DC quantification. SBU and CSE showed significantly higher mean µTBS and lower nanoleakage than XEN, regardless of the evaluation time and adhesion strategy. XEN-SE yielded the lowest degree of conversion. Therefore, adhesive properties of universal adhesives to dentin are material dependent, regardless of the adhesion strategy, exhibiting XEN a significantly worse performance than SBU.
One-year clinical performance of lithium disilicate versus resin composite CAD/CAM onlays
(Springer, 2020) Souza, Joana; Fuentes, Maria Victoria; Baena, Eugenia; Ceballos, Laura
To compare the 1-year clinical performance of lithium disilicate and resin composite CAD/CAM onlay restorations. Twenty patients that required two restorations in posterior teeth, with at least one cusp to be covered, received two onlays. One was made with IPS e.max CAD (Ivoclar-Vivadent) and the other with Lava Ultimate (3M Oral Care). Two blind observers evaluated the restorations at baseline and 1 year after the onlays were cemented, according to FDI criteria. At each recall, digital photographs, bite-wing radiographs and impressions of the restorations were taken for SEM evaluation of the interface. Results were analyzed by Mann-Whitney U and Wilcoxon tests (p < 0.05). At baseline and in the 1-year recall, both CAD/CAM materials exhibited excellent results in most criteria with similar esthetic, functional and biological properties (p > 0.05). However, deterioration in surface lustre (p = 0.020) and color match/translucency (p = 0.039) were detected for IPS e.max CAD onlays after 1-year. Under SEM evaluation, there were no statistically differences in micromorphological criteria at baseline nor after a year between IPS e.max CAD and Lava Ultimate onlays. Conclusion: After 1 year of clinical service IPS e.max CAD and Lava Ultimate onlays showed a similar clinical performance that needs to be confirmed in long-term evaluations.
Shear bond strength of a flash-free orthodontic adhesive system after thermal aging procedure
(Medicina Oral S.L, 2019) González-Serrano, Carlos; Baena q, Eugenia; Fuentes, Maria Victoria; Albadalejo, Alberto; Contreras-Míguez, Manuel; Lagravère, Manuel O; Ceballos, Laura
Background: The aim of this study was to compare the shear bond strength (SBS) of a flash-free and precoated orthodontic adhesive with a compomer orthodontic adhesive before and after thermocycling. The adhesive remnant index (ARI) was also determined for both adhesives. Material and methods: The adhesive remnant index (ARI) was also determined for both adhesives. Material and Methods: A total of 120 human premolars were randomly divided into two groups (n=60) according to the orthodontic adhesive used: APC Flash-Free Adhesive Coated Appliance System (APC FF) or Transbond PLUS Color Change Adhesive (TP), as control. A SBS test was performed and ARI value for each specimen was also assessed. Results were analyzed by two-way ANOVA and Tukey's Chi-square test (p<0.05). Results: SBS values were significantly influenced by thermocycling (p<0.01). Neither the orthodontic adhesive nor the interaction between adhesive and thermocycling statistically affected SBS results (p>0.05). Conclusions: APC FF and TP showed similar bond strength results. Thermocycling induced a significant decrease in SBS values for the two adhesives tested, without differences between 10,000 and 20,000 thermal cycles. Moreover, APC FF left less adhesive remnants on the enamel compared to TP. Key words:APC Flash-Free, APC cement, aging, orthodontics, resin cements.
The influence of irrigation solutions in the inorganic and organic radicular dentine composition
(Wiley, 2020-01-26) Barón , Marta; Morales, Victoria; Fuentes, Victoria; Linares, María; Escribano, Nuria; Ceballos, Laura
The aim of this study was to analyse changes in radicular dentine composition after different irrigation regimes using attenuated total reflectance Fouriertransform infrared spectroscopy (ATR-FTIR). Dentine slices from human single-root teeth were immersed in: (i) 5.25% sodium hypochlorite (NaOCl) for 1, 5 and 20 min; (ii) 17% EDTA for 1 min; and (iii) 5.25% NaOCl for 20 min, and 17% EDTA and 5.25% NaOCl, both for 1 min. Carbonate/mineral, Amide I/mineral and Amide III/CH2 ratios were determined using ATR-FTIR before and after immersion in the irrigation solutions tested. Results showed that 5.25% NaOCl significantly decreased carbonate/mineral, Amide I/mineral and Amide III/CH2 ratios (P < 0.05). Application of 17% EDTA produced no change in carbonate/mineral ratio (P = 0.120), while an increase in Amide I/mineral in apical third (P = 0.002) and Amide III/CH2 (P < 0.001) was observed. The combination of NaOCl, EDTA and NaOCl increased carbonate/mineral ratio in coronal third (P = 0.037), and Amide I/mineral (P = 0.003) and Amide III/CH2 (P = 0.001) ratios. In conclusion, irrigation solutions tested significantly affected radicular dentine composition.