Examinando por Autor "Galicia-Poblet, Gonzalo"

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    Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis
    (2023-02-02) Sánchez-Jaut, Sandra; Pérez-Benavente, Susana; Abad, Paloma; Méndez-Cuadro, Darío; Puyet, Antonio; Diez, Amalia; Galicia-Poblet, Gonzalo; Gómez-Domínguez, Elena; Moran-Jiménez, María J; Bautista, José M; Azcárate, Isabel G
    Iron overload caused by hereditary hemochromatosis (HH) increases free reactive oxygen species that, in turn, induce lipid peroxidation. Its 4-hydroxynonenal (HNE) by-product is a well-established marker of lipid peroxidation since it reacts with accessible proteins with deleterious consequences. Indeed, elevated levels of HNE are often detected in a wide variety of human diseases related to oxidative stress. Here, we evaluated HNE-modified proteins in the membrane of erythrocytes from HH patients and in organs of Hfe-/- male and female mice, a mouse model of HH. For this purpose, we used one- and two-dimensional gel electrophoresis, immunoblotting and MALDI-TOF/TOF analysis. We identified cytoskeletal membrane proteins and membrane receptors of erythrocytes bound to HNE exclusively in HH patients. Furthermore, kidney and brain of Hfe-/- mice contained more HNE-adducted protein than healthy controls. Our results identified main HNE-modified proteins suggesting that HH favours preferred protein targets for oxidation by HNE.