Examinando por Autor "Galindo, Miguel A"

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    Highly Stable Double‐Stranded DNA Containing Sequential Silver (I)‐Mediated 7‐Deazaadenine/Thymine Watson–Crick Base Pairs
    (Wiley, 2016-05-17) Santamaría‐Díaz, Noelia; Mendez‐Arriaga, Jose Manuel; Salas, Juan Manuel; Galindo, Miguel A
    The oligonucleotide d(TX)9, which consists of an octadecamer sequence with alternating non-canonical 7-deazaadenine (X) and canonical thymine (T) as the nucleobases, was synthesized and shown to hybridize into double-stranded DNA through the formation of hydrogen-bonded Watson–Crick base pairs. dsDNA with metal-mediated base pairs was then obtained by selectively replacing W-C hydrogen bonds by coordination bonds to central silver(I) ions. The oligonucleotide I adopts a duplex structure in the absence of Ag+ ions, and its stability is significantly enhanced in the presence of Ag+ ions while its double-helix structure is retained. Temperature-dependent UV spectroscopy, circular dichroism spectroscopy, and ESI mass spectrometry were used to confirm the selective formation of the silver(I)-mediated base pairs. This strategy could become useful for preparing stable metallo-DNA-based nanostructures.