Examinando por Autor "Linares, Rafael"

Mostrando 1 - 3 de 3

Characterization of a human Bid homologue protein from Gallus gallus
(Gene Section Functional Genomics, 2006) Díaz-Gil, Gema; Gómez-Esquer, Francisco; Agudo, David; Delcán, Jose; Martínez-Arribas, Fernando; Rivas, Carmen; Schneider, José; Palomar, Ma Angustias; Linares, Rafael
Bid protein, a member of the "BH3-only" subgroup of Bcl-2 family, plays a critical role in mammalian apoptosis regulation. In this study, we have cloned the chicken Bid gene, which encodes a 193 amino acid protein and shares 40% homology with human and mouse Bid proteins. Bid sequence comparison emphasises the conservation of both the functional domain BH3 and the proteolytic cleavage sites. An induction of apoptosis by chicken Bid and the cleavage of the protein, after TNF treatment, were also demonstrated. In addition, mRNA Bid expression was detected along all embryo stages and tissues examined, suggesting a role for this protein in the developmental process. This is the first report demonstrating the functionality of a "BH3-only" protein in chicken.
Effect of platelet rich plasma on sinus lifting: A randomized controlled clinical trial
(2011-12-02) Torres, Jesús; Tamimi, Faleh; Martínez, Pedro Pablo; Alkhraisat, Mohammad; Linares, Rafael; Hernandez, Gonzalo; Torres-Macho, Juan; López-Cabarcos, Enrique
6 7 Objective: The combination of anorganic bovine bone (ABB) with platelet rich plasma ABSTRACT 8 (PRP) has been widely used in bone regeneration procedures although its benefits are 9 10 11 12 13 14 15 16 17 18 19 20 a histology level and any adjunctive effects, we performed an ancillary study in 5 still unclear. The purpose of this study was to evaluate whether or not PRP improves the efficacy of ABB in sinus floor augmentation. In addition, we have investigated the effect of residual bone height and tobacco on implant survival in sinus augmentation procedures. Materials and Methods: 87 patients recruited for this study underwent 144 sinus floor augmentation procedures using ABB alone or ABB plus PRP (ABB+PRP) in a randomized clinical trial. A total of 286 implants were placed in the augmented bone, and their evolution was followed up for a period of 24 months. In order to investigate on 21 edentulous patients with symmetrical severely resorbed maxilla. In these patients, a 22 23 24 25 26 27 Results: Overall, 96.2% of ABB and 98.6% of ABB+PRP implant success was 28 bilateral sinus augmentation was randomly performed using ABB or ABB+PRP in a split-mouth design, and after 6 months, bone biopsies were taken from the implant sites for histological and histomorphometric analysis. obtained during the monitoring period and differences were not found between sites grafted with and without PRP in the 87 patients studied. Densitometry assessments and graft resorption were similar in both experimental groups. However, the histological and histomorphometrical analysis in the 5 edentulous patients revealed that bone 29 30 31 32 33 augmentation was significantly higher in sites treated with ABB+PRP (p¿0.05). 34 Another outcome from our study is that the lack of initial bone support (p¿0.05) and smoking (p=0.05) appeared to have a negative effect on the treatment success, that was accentuated when both circumstances coincided. 35 36 37 38 39 40 procedures. However, this study revealed that PRP can improve the osteoconductive 41 Conclusions: PRP is not a determining factor for implant survival in sinus lifting properties of ABB by increasing the volume of new bone formed. Moreover, in sinus augmentation procedures implant¿s survival rate seems more influenced by the residual bone height or by tobacco than by the type of bone graft. 42 43 44
Platelet rich plasma prevents titanium mesh exposure in alveolar ridge augmentation with anorganic bovine bone
(2011-12-02) Torres-Macho, Jesús; Tamimi, Faleh; Alkhraisat, Mohammad; Manchón Miralles, Ángel; Linares, Rafael; Prados-Frutos, Juan Carlos; López-Cabarcos, Enrique
4 5 6 7 Bone augmentation with the titanium mesh (Ti-mesh) technique is susceptible to a large ABSTRACT Objective: 8 rate of complications such as morbidity of bone graft donor site, and mesh exposure to 9 10 11 12 13 14 15 16 17 18 19 20 for clinical, radiographic and histological evaluation, and implant placement surgery. A the oral cavity. The purpose of this study was to evaluate the efficacy of anorganic bovine bone (ABB) in alveolar bone augmentation with the Ti-mesh technique, as an alternative to autologous bone grafts. In addition, we investigated the effect of platelet rich plasma (PRP) in preventing mesh exposure, by applying it to cover the Ti-mesh. Materials and Methods: The 30 patients recruited for this study underwent 43 alveolar bone augmentation with the Ti-mesh technique using in all of them ABB as graft material. In 15 patients the Ti-meshes were covered with PRP (PRP group) while in the other 15 the Ti-meshes were not (control group). After 6 months, patients were called 21 total of 97 implants were placed in the augmented bone and their evolution was 22 23 24 25 26 27 complications and bone formation. In the control group 28.5% of the cases suffered 28 followed up for a period of 24 months. Results: Significant differences were found between the two study groups in terms of from mesh exposure, while in the PRP group, no exposures were registered. Radiographic analysis revealed that bone augmentation was higher in the PRP group than in the control group. Interestingly, mesh exposure seemed to be a risk factor regarding graft resorption and failure. 29 30 31 32 33 Overall, 97.3% of implants placed in the control group and 100% of those placed in the 34 PRP group were successful during the monitoring period. We suggest that the positive effect of PRP on the Ti-mesh technique is due to its capacity to improve soft tissue healing, thereby protecting the mesh and graft material secured beneath the gingival tissues. 35 36 37 38 39 40 Conclusions: Alveolar bone augmentation using ABB alone in the Ti-mesh technique is 41 sufficient for implant rehabilitation. Besides, covering the Ti-meshes with PRP was a determining factor for avoiding mesh exposure in this study. Titanium mesh exposure provoked significant bone loss, but in most cases it did not affect the subsequent placement of implants.