Examinando por Autor "Mancuso, Edoardo"

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Bond Strength of Universal Adhesive/Resin Cement Combinations Relying on Touch-Cure Mechanisms
(MDPI, 2025-04-29) Forte, Annamaria; Baena, Eugenia; Mazzitelli, Claudia; Mancuso, Edoardo; D´Urso, Diego; Pellegrino, Gerardo; Ceballos , Laura; Mazzoni, Annalisa; Maravic, Tatjana
New dual-curing resin cements are constantly launched into the market to improve the bond strength between dentine and indirect restorations when light irradiation is limited by the restoration material. The present study evaluated the microshear bond strength (μSBS) of two dual-cured resin cements, Estecem II Plus (EP) and Variolink Esthetic DC (VAR), when resin composite or dentine substrates were conditioned with their corresponding universal adhesives, Tokuyama Universal Bond II (TUB) and Adhese Universal DC (ADH). The experimental groups (n = 20) were (1) TUB/EP light-cured, (2) TUB/EP self-cured, (3) ADH/VAR light-cured, and (4) ADH/VAR self-cured. A μSBS test was performed after 24 h (T0) or after thermocycling (TC), and failure modes were assessed. Data analysis was performed using three-way ANOVA and Tukey tests (p < 0.05). In composite, TUB/EP self-cured demonstrated the highest μSBS at T0 and TC. After TC, TUB/EP self-cured and ADH/VAR light-cured remained stable (p > 0.05). In dentine, TUB/EP light-cured was statistically superior to TUB/EP self-cured and ADH/VAR self-cured at T0. Thermocycling decreased the μSBS of light-curing groups. TUB/EP achieved optimal μSBS when the manufacturer's instructions were followed and the adhesive was self-cured, irrespective of the bonding substrate. However, ADH/VAR was more dependent on the type of bonding substrate than on the curing mode of the resin cement
Endogenous Enzymatic Activity in Dentin Treated with a Chitosan Primer
(MDPI, 2021) Maravic, Tatjana; Baena, Eugenia; Mazzitelli, Claudia; Uros, Josic; Mancuso, Edoardo; Checchi, Vittorio; Generali, Luigi; Ceballos, Laura; Breschi, Lorenzo; Mazzoni, Annalisa
The aim of this study was to evaluate the effect of different concentrations of chitosan polymer on dentinal enzymatic activity by means of gelatin and in situ zymography. Human dentin was frozen and ground in a miller. Dentin powder aliquots were demineralized with phosphoric acid and treated with three different concentrations of lyophilized chitosan polymer (1, 0.5 and 0.1 wt%) dissolved in distilled water. Dentin proteins were extracted from each experimental group and electrophoresed under non-reducing conditions in 10% SDS-PAGE containing fluorescein-labeled gelatin. After 48 h in the incubation buffer at 37 °C, proteolytic activity was registered under long-wave UV light scanner and quantified by using Image J software. Furthermore, additional teeth (n = 4) were prepared for the in situ zymographic analysis in unrestored as well as restored dentin pretreated with the same chitosan primers. The registered enzymatic activity was directly proportional to the chitosan concentration and higher in the restored dentin groups (p < 0.05), except for the 0.1% chitosan primer. Chitosan 0.1% only showed faint expression of enzymatic activity compared to 1% and 0.5% concentrations. Chitosan 0.1% dissolved in water can produce significant reduction in MMPs activity and could possibly contribute to bond strength preservation over time.
Tack-cure vs conventional polymerization methods: A systematic review on resin composite cements´properties
(Elsevier, 2025-06-18) D´Alessandro, Carlo; Baena, Eugenia; Josic, Uros; Maravic, Tatjana; Mancuso, Edoardo; Ceballos , Laura; Mazzoni , Annalisa; Blatz, Markus B; Breschi, Lorenzo; Mazzitelli, Claudia
Objectives: This systematic review aimed to address the following research question: "What is the effect of tack-cure compared to conventional polymerization methods on the resin composite cements' properties?". Data and sources: A comprehensive literature search was conducted across electronic databases, including Clarivate Analytics' Web of Science, Cochrane Library, EMBASE, PubMed, Scopus, and ProQuest, without language or publication date restrictions. In vitro studies comparing tack-cure (TC) with conventional polymerization methods (light-cure, LC, and/or self-cure, SC) were included. The risk of bias was assessed using the QUIN tool for in vitro studies. This systematic review was reported in accordance with PRISMA guidelines. Results: Sixteen relevant articles were included in this systematic review. According to the QUIN tool, 1 study was ranked as "low risk," 12 studies as "medium risk," and 3 studies as "high risk" of bias. The resin cements evaluated were mainly dual-cure (DC) adhesive/multistep and self-adhesive/one step materials, whereas only 1 universal cement was investigated. Overall, TC provided comparable or superior mechanical properties compared to LC, and consistently outperformed SC alone. Furthermore, TC facilitated excess cement removal and improved interface quality. However, outcomes varied depending on the type of resin cement, polymerization protocol, and evaluation method. Conclusions: TC can provide mechanical properties and interface quality comparable or superior to conventional polymerization methods, while facilitating excess cement removal. Clinical significance: TC appears to be a clinically useful technique that enhances handling without adversely affecting resin composite cements' properties. Clinicians should always follow manufacturers' instructions and consider cement-specific characteristics