Examinando por Autor "Navarro-Ranninger, Carmen"

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Enhanced Cytotoxicity and Reactivity of a Novel Platinum(IV) Family with DNA-Targeting Naphthalimide Ligands
(American Chemical Society, 2017-05-10) Navas, Francisco; Mendes, Filipa; Santos, Isabel; Navarro-Ranninger, Carmen; Cabrera, Silvia; Gómez Quiroga, Adoración
Pt(IV) complexes are known as prodrugs that can potentially overcome cisplatin limitations by slowing down its reactivity, and once reduced, act as the corresponding Pt(II) drugs. We report a new approach towards trans Pt(IV) complexes, conceived to afford nonconventional active trans Pt(II) complexes with dual targeting properties. The reduction of the complexes has been studied in the presence of ascorbic acid and glutathione, showing that different species are formed in the process. The interaction with DNA after reduction has been also studied and correlated to the formation of Pt(II) species. The cytotoxicity profile of the Pt(IV) complexes corroborated the rationale behind this approach.
Increasing DNA reactivity and in vitro antitumor activity of trans diiodido Pt(II) complexes with UVA light
(Elsevier Sciencie Inc., 2015-07-21) Navas, Francisco; Perfhal, Stefanie; Garino, Claudio; Salassa, Luca; Novakova, Olga; Navarro-Ranninger, Carmen; Bednarski, Patrick J.; Malina, Jaroslav; Gómez Quiroga, Adoración
Trans diiodido platinum(II) complexes bearing the same as well as different aliphatic amines (mixed-amines) have interesting biological activity; cytotoxicity and interactions with some important biological models have already been demonstrated. Herein we described the interaction of such compounds with ct-DNA, supercoiled and linearized plasmid DNA and 5-GMP. Interestingly, UV irradiation of these compounds results in an increase in reactivity towards DNA and 5-GMP in such model systems. Additionally, the cytotoxicity of the trans-Pt(II) complexes towards human cancer cells is noticeably increased when treatment is combined for 90 min with UVA-irradiation. With this work we provide evidence that trans diiodido compounds can be activated by UVlight over relatively short treatment times.