Examinando por Autor "Nieto Bona, M Paz"

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Early in vivo detection of denervation-induced atrophy by luminescence transient nanothermometry
(Wiley, 2023-11-27) Lifante, José; Moreno-Rupérez, Álvaro; Ximendes, Erwing; Marin, Riccardo; Priego, Teresa; López-Calderón, Asunción; Martín, Ana Isabel; Nieto Bona, M Paz; Nebot, Elena; Lifante-Pedrola, Ginés; Jaque, Daniel; Monge, Luis; Fernández, Nuria; Granado, Miriam
Denervation induces skeletal muscle atrophy due to the loss of control and feedback with the nervous system. Unfortunately, muscle atrophy only becomes evident days after the denervation event when it could be irreversible. Alternative diagnosis tools for early detection of denervation-induced muscle atrophy are, thus, required. In this work, we demonstrate how the combination of transient thermometry, a technique already used for early diagnosis of tumors, and infrared-emitting nanothermometers makes possible the in vivo detection of the onset of muscle atrophy at short (<1 day) times after a denervation event. The physiological reasons behind these experimental results have been explored by performing three dimensional numerical simulations based on the Pennes' bioheat equation. It is concluded that the alterations in muscle thermal dynamics at the onset of muscle atrophy are consequence of the skin perfusion increment caused by the alteration of peripheral nervous autonomous system. This work demonstrates the potential of infrared luminescence thermometry for early detection of diseases of the nervous system opening the venue toward the development of new diagnosis tools.
Time-Dependent Changes in Muscle IGF1-IGFBP5-PAPP System after Sciatic Denervation
(MDPI, 2023-09-14) Martín, Ana Isabel; Moreno-Rupérez, Álvaro; Nebot, Elena; Granado, Miriam; Jaque, Daniel; Nieto Bona, M Paz; López-Calderón, Asunción; Priego, Teresa
Denervation-induced muscle atrophy is a frequent cause of skeletal muscle diseases. However, the role of the most important muscle growth factor, insulin-like growth factor (IGF-1), in this process is poorly understood. IGF-1 activity is controlled by six IGF-1 binding proteins (IGFBPs). In skeletal muscle, IGFBP-5 seems to have an important role in atrophic processes. Furthermore, pappalysins (PAPP-A) modulate muscle growth by increasing IGF-1 bioavailability through IGFBP cleavage. We aimed to study the time-dependent changes in the IGF1-IGFBP5-PAPP system and its regulators in gastrocnemius muscle after sciatic denervation. Gastrocnemius atrophy and overexpression of IGF-1 was observed from day 3 post-denervation. The proteolytic factors measured were elevated from day 1 post-denervation onwards. Expression of both IGFBP-5 and pappalysins were increased on days 1 and 3. Subsequently, on days 7 to 14 pappalysins returned to control levels while IGFBP-5 remained elevated. The ratio IGFBP-5/PAPP-A was correlated with the main proteolytic markers. All data suggest that the initial increase of pappalysins could facilitate the IGF-1 action on muscle growth, whereas their subsequent decrease could lead to further muscle wasting.