Examinando por Autor "Ovejero-Paredes, Karina"

Mostrando 1 - 4 de 4

Albumin-Loaded Silica Nanomaterials Functionalized with Organotin(IV) Agents: Theranostic Materials Against Triple-Negative Breast Cance
(Wiley, 2024-07-11) García-Almodóvar, Victoria; Ovejero-Paredes, Karina; Díaz-García, Diana; Méndez-Arriaga, José M.; Prashar, Sanjiv; Filice, Marco; Gómez-Ruiz, Santiago
The current search for more effective and milder cancer treatments has led to the development of a wide variety of multifunctional nanoplatforms that are designed to both diagnose and treat cancer. In this study, the optimization of the synthesis of theranostic materials based on mesoporous silica nanoparticles (MSNs) functionalized with different cytotoxic (organotin(IV) compounds), imaging (fluorescein and/or indocyanine green), and targeting agents of interest, such as albumin (HA), is achieved by using different strategies. These systems shows good cytotoxic capacity against triple negative breast cancer (TNBC) cells (MDA-MB-231) in MTT (dimethylthiazolyl-diphenyl-tetrazolium bromide) assays and confocal analysis shows that the incorporation of HA as a potential active targeting molecule may enhance the cellular uptake of the nanomaterial, and thus, increasing its therapeutic potential. The analysis of the results and the effect of the imaging, targeting, and cytotoxic fragments should allow a more in-depth study of these materials in other in vitro and/or in vivo models
Amino acid-decorated mesoporous silica nanoparticles loaded with titanocene derivatives for targeted anticancer studies
(Wiley, 2024-04-21) Aondona Iorhemba, Michael; Ovejero-Paredes, Karina; Díaz-García, Diana; García-Almodóvar, Victoria; Ola Idris, Sulaiman; Adamu Shallangwa, Gideon; Abdulkadir, Ibrahim; Méndez-Arriaga, José M.; Prashar, Sanjiv; Filice, Marco; Gómez-Ruiz, Santiago
Nanostructured materials possess promising potential for cancer therapy through precise adjustment of their functionalization and physicochemical attributes. This study primarily focuses on the synthesis and characterization of mesoporous silica nanoparticles (MSN) that are functionalized with titanocene dichloride (a therapeutic agent) and one of several amino acids—cysteine, captopril, penicillamine, or methionine—utilizing 3-aminopropyltriethoxysilane (AP) as a linker. This synthesis yielded four innovative metallodrug-functionalized nanostructured materials (MSN-AP-Cys-Ti, MSN-AP-Cap-Ti, MSN-AP-Pen-Ti, and MSN-AP-Met-Ti), meticulously characterized using diverse analytical techniques such as X-ray diffraction (XRD), X-ray fluorescence (XRF), diffuse reflectance ultraviolet–visible (DR UV–Vis), Fourier transform infrared (FTIR), solid-state nuclear magnetic resonance (NMR) spectroscopy, and transmission electron microscopy (TEM). The textural properties of the nanomaterials post-functionalization displayed slight modifications, confirming the successful integration of the therapeutic agents. Evaluation of cytotoxicity in the breast cancer cell line MDA-MB-231, with the healthy cell line Hek-293T as control via MTT assays, revealed the active nature of the functionalized silica-based materials. The viability of both cell lines indicated a concentration-dependent response to the materials. Among the tested systems, cysteine and captopril exhibited the highest activity concerning IC50 relative to material concentration. The enhanced biological activity of higher functionalized nanosystems suggests a favorable cell internalization facilitated by the amino acid fragment. Additionally, qualitative DNA binding studies hinted at potential DNA adsorption on the surface of the metallodrug-functionalized nanomaterials, forming DNA adducts where a strand of DNA covalently bonds to the metallodrug moiety. This was deduced from the hypsochromic shift in absorbance of the characteristic π–π* and n–π* transitions in DNA, which occurred from 1.01 to 0.76 and 1.26–0.19 following drug (MSN-AP-Cap-Ti) interaction
Organotin(IV)-Decorated Graphene Quantum Dots as Dual Platform for Molecular Imaging and Treatment of Triple Negative Breast Cancer
(Wiley, 2023) Gómez, Jénnifer; Ovejero-Paredes, Karina; Méndez-Arriaga, José Manuel; Pizúrová, Naděžda; Filice, Marco; Zajíčková, Lenka; Prashar, Sanjiv; Gómez-Ruiz, Santiago
The pharmacological activity of organotin(IV) complexes in cancer therapy is well recognized but their large applicability is hampered by their poor water solubility. Hence, carbon dots, in particular nitrogen-doped graphene quantum dots (NGQDs), may be a promising alternative for the efficient delivery of organotin(IV) compounds as they have a substantial aqueous solubility, a good chemical stability, and non-toxicity as well as a bright photoluminescence that make them ideal for theranostic applications against cancer. Two different multifunctional nanosystems have been synthesized and fully characterized based on two fragments of organotin-based cytotoxic compounds and 4-formylbenzoic acid (FBA), covalently grafted onto the NGQDs surface. Subsequently, an in vitro determination of the therapeutic and theranostic potential of the achieved multifunctional systems was carried out. The results showed a high cytotoxic potential of the NGQDs-FBA-Sn materials against breast cancer cell line (MDA-MB-231) and a lower effect on a non-cancer cell line (kidney cells, HEK293T). Besides, thanks to their optical properties, the dots enabled their fluorescence molecular imaging in the cytoplasmatic region of the cells pointing towards a successful cellular uptake and a release of the metallodrug inside cancer cells (NGQDs-FBA-Sn).
Synthesis of a theranostic platform based on fibrous silica nanoparticles for the enhanced treatment of triple-negative breast cancer promoted by a combination of chemotherapeutic agents
(Elsevier, 2022) Ovejero-Paredes, Karina; Díaz-García, Diana; Mena-Palomo, Irene; Marciello, Marzia; Lozano-Chamizo, Laura; Luengo Morato, Yurena; Prashar, Sanjiv; Gómez-Ruiz, Santiago; Filice, Marco
A new series of theranostic silica materials based on fibrous silica particles acting as nanocarriers of two different cytotoxic agents, namely, chlorambucil and an organotin metallodrug have been prepared and structurally characterized. Besides the combined therapeutic activity, these platforms have been decorated with a targeting molecule (folic acid, to selectively target triple negative breast cancer) and a molecular imaging agent (Alexa Fluor 647, to enable their tracking both in vitro and in vivo). The in vitro behaviour of the multifunctional silica systems showed a synergistic activity of the two chemotherapeutic agents in the form of an enhanced cytotoxicity against MDA-MB-231 cells (triple negative breast cancer) as well as by a higher cell migration inhibition. Subsequently, the in vivo applicability of the siliceous nanotheranostics was successfully assessed by observing with in vivo optical imaging techniques a selective tumour accumulation (targeting ability), a marked inhibition of tumour growth paired to a marked antiangiogenic ability after 13 days of systemic administration, thus, confirming the enhanced theranostic activity. The systemic nanotoxicity was also evaluated by analyzing specific biochemical markers. The results showed a positive effect in form of reduced cytotoxicity when both chemotherapeutics are administered in combination thanks to the fibrous silica nanoparticles. Overall, our results confirm the promising applicability of these novel silica-based nanoplatforms as advanced drug-delivery systems for the synergistic theranosis of triple negative breast cancer.