Examinando por Autor "Piera, Alejandro"

Mostrando 1 - 2 de 2

A successful method for phycocyanin extraction from Arthrospira platensisusing [Emim] [EtSO4] ionic liquid
(Wiley, 2021) Sánchez-Laso, Jennifer; Piera, Alejandro; Vicente, Gemma; Bautista, Luis Fernando; Rodríguez, Rosalía; Espada, Juan J.
Research into the extraction of phycobiliprotein from Arthrospira platensis is currently ongoing. Most traditional methods of phycobiliprotein extraction include the use of organic solvents, which negatively affect the sustainability of the process. Ionic liquids (ILs) are a promising alternative for phycobiliprotein extraction due to their properties as green solvents. In the present work an imidazolium-based IL and sonication were studied for phycobiliprotein and carbohydrate extraction. A factorial experimental design was used to optimize the amount of extracted phycocyanin. The maximum extraction yield was achieved by using low biomass/solvent ratios combined with high IL/water ratios and sonication powers, and long operation times. The recovery of IL was studied under these conditions using a dialysis-based process to separate the IL from the extracted phycobiliproteins. The results reveal the possibility of using the recovered IL for seven consecutive extraction cycles with an acceptable phycocyanin extraction amount, from 75 mg g−1 (fresh IL) to 60 mg g−1 (7 cycles of reused IL). The use of [Emim] [EtSO4] + ultrasound is a promising alternative for phycocyanin extraction, enhancing the results obtained with other IL in terms of extracted phycocyanin and the number of times the IL can be reused.
Optimised phycoerythrin extraction method from Porphyridium sp. combining imidazolium-based ionic liquids
(Elsevier, 2024-07-30) Piera, Alejandro; Espada, Juan J.; Morales, Victoria; Rodríguez, Rosalía; Vicente, Gemma; Bautista, Luis Fernando
Phycoerythrin (PE) extraction from Porphyridium sp. was studied employing ultrasound-assisted extraction combined with aqueous mixtures of two imidazolium-based ionic liquids (ILs) simultaneously, marking a significant novelty. A face-centred central composite design and response surface optimised PE yield (EPE), considering the effects of ionic liquid concentration (IL), [Emim][EtSO4]/[Bmim][EtSO4] mass ratio (E/B), biomass concentration (BM), and time (t). Improvements in EPE by 300 % and 115 % were achieved compared to a phosphate buffer solution and the freeze-thaw method, respectively. Temperature and pH effects were examined independently, leading to the determination of optimal operating conditions: BM = 10 mg mL−1, IL = 18.6 wt%, E/B = 0.78/0.22, t = 10 min, T = 35 °C, and pH = 7.5. Results indicated the potential for reusing the ILs for at least five consecutive extraction cycles, maintaining an EPE of 94.2 % compared to fresh ones. This underscores the success and innovation of the developed technology in enhancing PE extraction from Porphyridium sp