Examinando por Autor "Villamil, John A."

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Using inorganic acids to stop purple phototrophic bacteria metabolism improves PHA recovery at a large scale
(Springer, 2023) Srivastava, Pratiksha; Villamil, John A.; Melero, Juan A.; Martínez, Fernando; Puyol, Daniel
Polyhydroxyalkanoate (PHA) production at a large scale by purple phototrophic bacteria (PPB) is hindered due to high production costs and limited recovery due to its consumption during starvation periods. The present study identifed costefective inorganic acids as inactivation methods for PPB to obtain higher PHA recovery. The study was performed on reactors of diferent scales (10 L and 0.5 L) to grow PPB and recover PHA subsequently. The permanent feast strategy was adopted to obtain higher PHA in an anaerobic environment. As a result, the study achieved 33% (dry weight) PHA recovery using inorganic acid inactivation, while formaldehyde inactivation (traditional method) achieved signifcantly lower PHA recovery (20% only). The results from inorganic acid inactivation were further examined for their stability. The samples were stable even after day 14, and the PHA recovery was the same as on day 0. This pioneering study shows that inorganic acids can be used to inactivate the PPB metabolism to obtain higher PHA recovery; inorganic acid inactivation could be economical for large-scale PHA production.