Examinando por Autor "Bujanda, Luis"

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Colorectal cancer risk factors in patients with serrated polyposis syndrome: a large multicentre study
(BMJ Publishing Group Ltd (& BSG), 2016-11) Carballal, Sabela; Rodríguez-Alcalde, Daniel; Moreira, Leticia; Hernández, Luis; Rodríguez, Lorena; Rodríguez-Moranta, Francisco; Gonzalo, Victoria; Bujanda, Luis; Bessa, Xavier; Poves, Carmen; Cubiella, Joaquín; Castro, Inés; González, Mariano; Moya, Eloísa; Oquiñena, Susana; Clofent, Joan; Quintero, Enrique; Esteban, Pilar; Piñol, Virginia; Fernández, Francisco Javier; Jover, Rodrigo; Cid, Lucía; López-Cerón, María; Cuatrecasas, Miriam; López-Vicente, Jorge; Leoz, María Liz; Rivero-Sánchez, Liseth; Castells, Antoni; Pellisé, María; Balaguer, Francesc
Objective: Serrated polyposis syndrome (SPS) is associated with an increased colorectal cancer (CRC) risk, although the magnitude of the risk remains uncertain. Whereas intensive endoscopic surveillance for CRC prevention is advised, predictors that identify patients who have high CRC risk remain unknown. We performed a multicentre nationwide study aimed at describing the CRC risk in patients with SPS and identifying clinicopathological predictors independently associated with CRC. Design: From March 2013 through September 2014, patients with SPS were retrospectively recruited at 18 Spanish centres. Data were collected from medical, endoscopy and histopathology reports. Multivariate logistic regression was performed to identify CRC risk factors. Results: In 296 patients with SPS with a median follow-up time of 45 months (IQR 26–79.7), a median of 26 (IQR 18.2–40.7) serrated polyps and 3 (IQR 1–6) adenomas per patient were detected. Forty-seven patients (15.8%) developed CRC at a mean age of 53.9±12.8, and 4 out of 47 (8.5%) tumours were detected during surveillance (cumulative CRC incidence 1.9%). Patients with >2 sessile serrated adenomas/polyps (SSA/Ps) proximal to splenic flexure and ≥1 proximal SSA/P with high-grade dysplasia were independent CRC risk factors (incremental OR=2, 95% CI 1.22 to 3.24, p=0.006). Patients with no risk factors showed a 55% decrease in CRC risk (OR=0.45, 95% CI 0.24 to 0.86, p=0.01). Conclusions: Patients with SPS have an increased risk of CRC, although lower than previously published. Close colonoscopy surveillance in experienced centres show a low risk of developing CRC (1.9% in 5 years). Specific polyp features (SSA/P histology, proximal location and presence of high-grade dysplasia) should be used to guide clinical management.
Proteomic Profiling of Extracellular Vesicles in Inflammatory Bowel Diseases
(MDPI, 2025-01-09) Baldán-Martín, Montse; Azkargorta , Mikel; Lapitz, Ainhoa; Ortega Moreno, Lorena; Iloro, Ibon; Fernández-Tomé, Samuel; Arbelaiz, Ander; Escobes, Iraide; Marín, Alicia C.; Bernardo, David; Bujanda, Luis; Bañales, Jesús M.; Elortza, Félix; Gisbert, Javier P.; Chaparro, María
Background: The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. Aims: To characterize the proteomic composition of serum EVs in patients with CD and UC to identify biomarkers and molecular pathways associated with pathogenesis and activity. Methods: Serum EVs were enriched and analyzed in patients with quiescent CD, active CD (aCD), quiescent UC, active UC (aUC), and healthy controls (HCs) (n = 30 per group). All groups were matched for age and sex. Disease activity was assessed by ileocolonoscopy and categorized based on the SES-CD (CD) and the endoscopic sub-score of the Mayo Score (UC). EVs were enriched by ultracentrifugation, and their size and concentration were determined by nanoparticle tracking analysis. The expression of CD63, CD81, and CD9 was determined using Western blotting. Proteomic analysis was performed by label-free nano-LC MS/MS. Results: A total of 324 proteins were identified; 60 showed differential abundance in CD-HC, 34 in UCHC, and 21 in CD-UC. Regarding disease activity, the abundance of 58 and 32 proteins was altered in aCD-HC and aUC-HC, respectively. Functional analyses revealed that proteins associated with aCD were involved in immune regulation, whereas those linked to aUC were enriched in oxidative stress. Conclusions: We have identified expressed proteins between EVs from patients with CD and UC, depending on the presence of disease, the disease type, and the disease activity. These proteins are potential candidates as disease biomarkers and open new research avenues to better understand these conditions.