Green extraction approach based on μSPEed® followed by HPLC-MS/MS for the determination of atropine and scopolamine in tea and herbal tea infusions
A high throught methododology based on a green extraction technique, μSPEed®, followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been proposed for the analysis of atropine and scopolamine in tea and herbal tea infusions. For this, a digiVOL® Digital Syringe was used with different sorbents and working conditions to obtain a fast and efficient μSPEed® extraction. The best performance was achieved with a PS/DVB sorbent phase, sample loading of 5 x 500 μL and elution with 2 x 100 μL aliquots of methanol. The strategy based on μSPEed® followed by HPLC-MS/MS was validated, attaining quantitation limits lower than 0.15 ng mL-1 and recoveries between 94 -106% for both analytes and applied to seventeen tea and herbal tea infusions. Fourteen infusions showed contamination with one or both analytes above the maximum content legislated (sum of atropine and scopolamine < 0.2 ng mL-1).
This research was funded by MCIU/AEI/FEDER, UE, project number RTI2018-094558-B-I00 (EVALKALIM). This work was also funded by FCT-Fundação para a Ciência e a Tecnologia through the CQM Base Fund - UIDB/00674/2020, and Programmatic Fund - UIDP/00674/2020, and by ARDITI-Agência Regional para o Desenvolvimento da Investigação Tecnologia e Inovação, through the project M1420-01-0145-FEDER-000005 - Centro de Química da Madeira - CQM+ (Madeira 14-20 Program) and by the project M1420–09–5369-FSE-000001. The authors also acknowledge the financial support from Fundação para a Ciência e Tecnologia and Madeira 14-2020 program to the Portuguese Mass Spectrometry Network through PROEQUIPRAM program, M14-20 M1420-01-0145-FEDER-000008. Lorena González-Gómez would like to thank the Rey Juan Carlos University for providing her a mobility grant to carry out a pre-doctoral stay in the CQM – Centro de Química da Madeira.
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