Show simple item record

A cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris.

dc.contributor.authorChen, Hongxin
dc.contributor.authorQuintana, Julia
dc.contributor.authorKovalchuk, Andriy
dc.contributor.authorUbhayasekera, Wimal
dc.contributor.authorAsiegbu, Fred O
dc.date.accessioned2023-12-13T12:25:50Z
dc.date.available2023-12-13T12:25:50Z
dc.date.issued2015-09-15
dc.identifier.citationChen, H., Quintana, J., Kovalchuk, A., Ubhayasekera, W., Asiegbu, F.O., 2015. A cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris. Fungal genetics and biology : FG & B 84, 41–51. https://doi.org/10.1016/j.fgb.2015.09.007es
dc.identifier.urihttps://hdl.handle.net/10115/27226
dc.descriptionThis work was supported by the Academy of Finland (Finland). Supports from Integrative Life Science Doctoral Program (ILS) (Finland) to Hongxin Chen are gratefully acknowledged.es
dc.description.abstractThe cerato-platanin family is a group of small secreted cysteine-rich proteins exclusive for filamentous fungi. They have been shown to be involved in the interactions between fungi and plants. Functional characterization of members from this family has been performed mainly in Ascomycota, except Moniliophthora perniciosa. Our previous phylogenetic analysis revealed that recent gene duplication of cerato-platanins has occurred in Basidiomycota but not in Ascomycota, suggesting higher functional diversification of this protein family in Basidiomycota than in Ascomycota. In this study, we identified three cerato-platanin homologues from the basidiomycete conifer pathogen Heterobasidion annosum sensu stricto. Expression of the homologues under various conditions as well as their roles in the H. annosum s.s.-Pinus sylvestris (Scots pine) pathosystem was investigated. Results showed that HaCPL2 (cerato-platanin-like protein 2) had the highest sequence similarity to cerato-platanin from Ceratocystis platani and hacpl2 was significantly induced during nutrient starvation and necrotrophic growth. The treatment with recombinant HaCPL2 induced cell death, phytoalexin production and defense gene expression in Nicotiana tabacum. Eliciting and cell death-inducing ability accompanied by retardation of apical root growth was also demonstrated in Scots pine seedlings. Our results suggest that HaCPL2 might contribute to the virulence of H. annosum s.s. by promoting plant cell death.es
dc.publisherElsevieres
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleA cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris.es
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/j.fgb.2015.09.007es
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccesses


Files in this item

This item appears in the following Collection(s)

Show simple item record

CC BY-NC-ND 4.0Except where otherwise noted, this item's license is described as CC BY-NC-ND 4.0