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Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana

dc.contributor.authorJiang, Xi
dc.contributor.authorCoroian, Diana
dc.contributor.authorBarahona, Emma
dc.contributor.authorEchavarri-Erasun, Carlos
dc.contributor.authorCastellanos-Rueda, Rocío
dc.contributor.authorEseverri, Álvaro
dc.contributor.authorAznar-Moreno, Jose
dc.contributor.authorBuren, Stefan
dc.contributor.authorRubio, Luis Manuel
dc.date.accessioned2023-12-26T20:54:29Z
dc.date.available2023-12-26T20:54:29Z
dc.date.issued2022-06-13
dc.identifier.issn2161-2129
dc.identifier.urihttps://hdl.handle.net/10115/27913
dc.descriptionEste estudio fue respaldado, en su totalidad o en parte, por la Fundación Bill y Melinda Gates (OPP1143172 e INV-005889).es
dc.description.abstractEngineering plants to synthesize nitrogenase and assimilate atmospheric N2 will reduce crop dependency on industrial N fertilizers. This technology can be achieved by expressing prokaryotic nitrogen fixation gene products for the assembly of a functional nitrogenase in plants. NifB is a critical nitrogenase component since it catalyzes the first committed step in the biosynthesis of all types of nitrogenase active-site cofactors. Here, we used a library of 30 distinct nifB sequences originating from different phyla and ecological niches to restore diazotrophic growth of an Azotobacter vinelandii nifB mutant. Twenty of these variants rescued the nifB mutant phenotype despite their phylogenetic distance to A. vinelandii. Because multiple protein interactions are required in the iron-molybdenum cofactor (FeMo-co) biosynthetic pathway, the maturation of nitrogenase in a heterologous host can be divided in independent modules containing interacting proteins that function together to produce a specific intermediate. Therefore, nifB functional modules composed of a nifB variant, together with the A. vinelandii NifS and NifU proteins (for biosynthesis of NifB [Fe4S4] clusters) and the FdxN ferredoxin (for NifB function), were expressed in Nicotiana benthamiana chloroplasts and mitochondria. Three archaeal NifB proteins accumulated at high levels in soluble fractions of chloroplasts (Methanosarcina acetivorans and Methanocaldococcus infernus) or mitochondria (M. infernus and Methanothermobacter thermautotrophicus). These NifB proteins were shown to accept [Fe4S4] clusters from NifU and were functional in FeMo-co synthesis in vitro. The accumulation of significant levels of soluble and functional NifB proteins in chloroplasts and mitochondria is critical to engineering biological nitrogen fixation in plants.es
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectFeMo-cofactores
dc.subjectmetalloproteinses
dc.subjectnifes
dc.subjectnitrogen fixationes
dc.subjectsynthetic biologyes
dc.titleFunctional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamianaes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1128/mbio.00268-22es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses


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Atribución 4.0 InternacionalExcept where otherwise noted, this item's license is described as Atribución 4.0 Internacional