dc.identifier.citation | Martínez-García I, Gaona-Scheytt C, Morante-Zarcero S, Sierra I. Development of a Green, Quick, and Efficient Method Based on Ultrasound-Assisted Extraction Followed by HPLC-DAD for the Analysis of Bioactive Glycoalkaloids in Potato Peel Waste. Foods. 2024; 13(5):651. https://doi.org/10.3390/foods13050651 | es |
dc.description.abstract | α-Solanine and α-chaconine are the two most predominant glycoalkaloids (GAs) present
in potato. Potato peel contains a high concentration of GAs, which are especially interesting for
application in the pharmaceutical industry due to their different beneficial properties (such as
anticarcinogenic, anti-inflammatory, antiallergic, antipyretic, antiviral, fungicide, and antibiotic
activities, among others); so, potato peel waste can be valorized by extracting these biologically
active compounds. For this, a green, quick, and efficient miniaturized analytical approach based
on ultrasound-assisted extraction (UAE) combined with HPLC-DAD was developed to quantify
α-solanine and α-chaconine in potato peel. Some parameters of the extraction were optimized,
including the extraction method, the type of solvent, and the sample/solvent ratio, by a three-factor,
three-level (33
) full factorial experimental design. The optimal extraction conditions were obtained
with UAE using methanol as a solvent and a sample/solvent ratio of 1:10 (w/v, g/mL). The analytical
greenness metric for sample preparation (AGREEprep) tool was used to assess the greenness of the
methods used. The tool revealed an acceptable green analysis, with 0.61 points. The method was
validated and applied to the evaluation of GAs in the peel of 15 commercial varieties of potato. The
amount of glycoalkaloids found in the samples evaluated ranged from 143 to 1273 mg/kg and from
117 to 1742 mg/kg dry weight for α-solanine and α-chaconine, respectively. These results reveal the
important variability that exists between potato varieties; so, their analysis is of great importance
to select the most suitable ones for biovalorization (e.g., the Amandine and Rudolph varieties, with
around 3000 mg/kg, in total, of both GAs). To provide higher stability to the peel during storage,
freeze-drying or a medium-temperature drying process resulted preferable to avoid GA degradation.
Overall, this study will contribute to the expansion of the future biovalorization of potato peel waste
as well as provide a powerful analytical tool for GA analysis. | es |