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Effect of phorbol esters on noradrenaline release from cerebral arteries

dc.contributor.authorBalfagón, Gloria
dc.contributor.authorde Sagarra, M Rosa
dc.contributor.authorBarrús, M Teresa
dc.contributor.authorArribas, Silvia
dc.contributor.authorCapilla, M Isabel
dc.contributor.authorMarín, Jesús
dc.date.accessioned2024-07-08T11:55:01Z
dc.date.available2024-07-08T11:55:01Z
dc.date.issued1989-01-16
dc.identifier.citationGloria Balfagon, M. Rosa de Sagarra, M. Teresa Barrus, Silvia Arrivas, M. Isabel Capilla, Jesus Marin, Effect of phorbol esters on noradrenaline release from cerebral arteries, Brain Research, Volume 477, Issues 1–2, 1989, Pages 196-201, ISSN 0006-8993, https://doi.org/10.1016/0006-8993(89)91407-8.es
dc.identifier.issn0006-8993 (print)
dc.identifier.issn1872-6240 (online)
dc.identifier.urihttps://hdl.handle.net/10115/37114
dc.description.abstractAbstract The effect of phorbol 12-myristate, 13-acetate (PMA), an activator of protein kinase C, on noradrenaline (NA) release from cat cerebral arteries preincubated with [3H]NA was investigated in order to examine the role of that enzyme in this secretion. PMA (3 μM) potentiated tritium release elicited by electrical stimulation (2 Hz, 0.3 ms) without modification of spontaneous secretion, whereas 4α-phorbol 12,13 didecanoate (3 μM), an inactive compound, had no effect. Tritium release evoked by tyramine was not modified by PMA. The electrically evoked tritium secretion was reduced by clonidine (1 μM) or B-HT 920 (0.1 μM), α2-adrenoceptor agonists, and unaffected by yohimbine (1 μM), an α2-adrenoceptor antagonist. The presence of clonidine, B-HT 920 or yohimbine reduced the action of PMA. The facilitatory effect of PMA was not increased by the Ca2+ ionophore A23187 (5 μM). These results suggest that: (1) protein kinase C of perivascular adrenergic nerve endings participates in the exocytotic release of NA; (2) the effects of PMA could be partially due to an interference with prejunctional autoinhibitory α2-adrenoceptors, and (3) the increase of intracellular Ca2+ produced by A23187 appears to be inadequate for potentiating the action of PMA.es
dc.language.isoenges
dc.publisherElsevieres
dc.subjectcerebral artery; noradrenaline release; alpha2-adrenoceptor agonist; alpha2-adrenoceptor antagonist; protein kinase C; phorbol ester; ionophore A23187es
dc.titleEffect of phorbol esters on noradrenaline release from cerebral arterieses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/0006-8993(89)91407-8es
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccesses


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