Mitochondrial Fusion Is Increased by the Nuclear Coactivator PGC-1ß
dc.contributor.author | Liesa, Marc | |
dc.contributor.author | Borda-d'Água, Bárbara | |
dc.contributor.author | Medina-Gómez, Gema | |
dc.contributor.author | Lelliott, Christopher J. | |
dc.contributor.author | Paz, José Carlos | |
dc.contributor.author | Rojo-Álvarez, José Luis | |
dc.contributor.author | Palacín, Manuel | |
dc.contributor.author | Vidal-Puig, Antonio | |
dc.contributor.author | Zorzano, Antonio | |
dc.date.accessioned | 2010-02-22T17:10:53Z | |
dc.date.available | 2010-02-22T17:10:53Z | |
dc.date.issued | 2008-10-31 | |
dc.description.abstract | There is no evidence to date on whether transcriptional regulators are able to shift the balance between mitochondrial fusion and fission events through selective control of gene expression. METHODOLOGY/PRINCIPAL FINDINGS: Here, we demonstrate that reduced mitochondrial size observed in knock-out mice for the transcriptional regulator PGC-1beta is associated with a selective reduction in Mitofusin 2 (Mfn2) expression, a mitochondrial fusion protein. This decrease in Mfn2 is specific since expression of the remaining components of mitochondrial fusion and fission machinery were not affected. Furthermore, PGC-1beta increases mitochondrial fusion and elongates mitochondrial tubules. This PGC-1beta-induced elongation specifically requires Mfn2 as this process is absent in Mfn2-ablated cells. Finally, we show that PGC-1beta increases Mfn2 promoter activity and transcription by coactivating the nuclear receptor Estrogen Related Receptor alpha (ERRalpha). CONCLUSIONS/SIGNIFICANCE: Taken together, our data reveal a novel mechanism by which mammalian cells control mitochondrial fusion. In addition, we describe a novel role of PGC-1beta in mitochondrial physiology, namely the control of mitochondrial fusion mainly through Mfn2. | es |
dc.description.departamento | Bioquímica, Fisiología y Genética Molecular | |
dc.description.sponsorship | This study was supported by research grants from the Ministerio de Educación y Cultura (SAF 2005-00445; SAF2008-03803), Grant 2005SGR00947 from the Generalitat de Catalunya, and CIBERDEM (Instituto de Salud Carlos III). A.Z. is the recipient of a Science Intensification Award from the University of Barcelona. M.L. is the recipient of a predoctoral fellowship from the Ministerio de Educación y Cultura, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es |
dc.identifier.citation | Liesa M et al. (2008) Mitochondrial Fusion Is Increased by the Nuclear Coactivator PGC-1b. PLoS One. 2008;3(10):e3613. Epub 2008 Oct 31. | es |
dc.identifier.doi | 10.1371/journal.pone.0003613 | es |
dc.identifier.other | PMID- 18974884 | |
dc.identifier.uri | http://hdl.handle.net/10115/3352 | |
dc.language.iso | en | es |
dc.publisher | PLoS ONE | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
dc.subject | Biología y Biomedicina | es |
dc.subject.unesco | 3209.90 Farmacología Experimental | es |
dc.title | Mitochondrial Fusion Is Increased by the Nuclear Coactivator PGC-1ß | es |
dc.type | info:eu-repo/semantics/article | es |