Application of a hybrid ordered mesoporous silica as sorbent for solid-phase multi-residue extraction of veterinary drugs in meat by ultra-high-performance liquid chromatography coupled to ion-trap tandem mass spectrometry

dc.contributor.authorCasado, Natalia
dc.contributor.authorMorante-Zarcero, Sonia
dc.contributor.authorPérez-Quintanilla, Damián
dc.contributor.authorSierra, Isabel
dc.date.accessioned2016-07-07T07:02:06Z
dc.date.available2016-07-07T07:02:06Z
dc.date.issued2016-06
dc.description.abstractA quick, sensitive and selective analytical reversed-phase multi-residue method using ultra-high performance liquid chromatography coupled to an ion-trap mass spectrometry detector (UHPLC-IT-MS/MS) operating in both positive and negative ion mode was developed for the simultaneous determination of 23 veterinary drug residues (ß-blockers, ß-agonists and Non-Steroidal Anti-inflammatory Drugs (NSAIDs)) in meat samples. The sample treatment involved a liquid-solid extraction followed by a solid-phase extraction (SPE) procedure. SBA-15 type mesoporous silica was synthetized and modified with octadecylsilane, and the resulting hybrid material (denoted as SBA-15-C18) was applied and evaluated as SPE sorbent in the purification of samples. The materials were comprehensively characterized, and they showed a high surface area, high pore volume and a homogeneous distribution of the pores. Chromatographic conditions and extraction procedure were optimized, and the method was validated according to the Commission Decision 2002/657/EC. The method detection limits (MDLs) and the method quantification limits (MQLs) were determined for all the analytes in meat samples and found to range between 0.01-18.75 ¿g/Kg and 0.02-62.50 ¿g/Kg, respectively. Recoveries for 15 of the target analytes ranged from 71-98%. In addition, for comparative purpose SBA-15-C18 was evaluated towards commercial C18 amorphous silica. Results revealed that SBA-15-C18 was clearly more successful in the multi-residue extraction of the 23 mentioned analytes with higher recovery values. The method was successfully tested to analyze prepacked preparations of mince bovine meat. Traces of propranolol, ketoprofen and diclofenac were detected in some samples.es
dc.identifier.citationJournal of Chromatography A, 2016.es
dc.identifier.doi10.1016/j.chroma.2016.06.077es
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10115/14000
dc.language.isoenges
dc.publisherElsevieres
dc.relation.projectIDS2013/ABI-3028es
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectMaterialeses
dc.subjectSolid-phase extractiones
dc.subjectmulti-residue methodes
dc.subjectVeterinary drugses
dc.subjectultra-high performance liquid chromatographyes
dc.subjectSBA-15 hybrid mesoporous silicaes
dc.subject.unesco23 Químicaes
dc.titleApplication of a hybrid ordered mesoporous silica as sorbent for solid-phase multi-residue extraction of veterinary drugs in meat by ultra-high-performance liquid chromatography coupled to ion-trap tandem mass spectrometryes
dc.typeinfo:eu-repo/semantics/articlees

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